Skip to main content
. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Nat Chem Biol. 2012 Sep 16;8(11):913–919. doi: 10.1038/nchembio.1070

Figure 2.

Figure 2

Mass spectrometric characterization of two novel hydrophobic small molecule-RNA conjugates. (a) Negative ion mode MS/MS of the two unknown nucleotides reveals a dinucleotide structure with uracil and two unknown nucleobases of 307.171 and 351.161 Da. (b and c) Positive mode MS/MS of the unknown nucleobases of 307.171 Da (b) and 351.161 Da (c) from unlabeled as well as 13C- and 15N-labeled RNA. The spectra of the unlabeled RNA are shown in red, the 13C-labeled samples are shown in black, and the 15N-labeled samples are shown in green. The +10 Da shifts, indicating 10 carbon atoms, of the geranyl fragment in the 13C-labeled RNA spectra are shown. (d) Proposed structures for the individual ion fragments observed in the MS/MS experiments.