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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Nat Chem Biol. 2012 Sep 16;8(11):913–919. doi: 10.1038/nchembio.1070

Figure 4.

Figure 4

Characterization of geranylated cellular RNAs. (a) MS analysis of individual tRNAs isolated from cells reveals mnm5ges2U to be present on tRNAGluUUC, tRNALysUUU, and (to a lesser extent) tRNAGlnUUG, and cmnm5ges2U to be present on tRNAGlnUUG. The same analysis is shown from tRNAAsnGUU as a negative control. (b) MS/MS fragmentation of the T1 digestion product of tRNAGluUUC reveals that the geranylated nucleotide is U34.