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. 2012 Sep 4;22(11):1576–1592. doi: 10.1038/cr.2012.129

Figure 5.

Figure 5

Mutant PI4KIIIβ rescues the effect of enviroxime on CVB3 replication. (A) Replication rescue experiment. BGM cells were transfected with HA-tagged PI4KIIIβ-wt, PI4KIIIβ-Y583M, or as negative controls the kinase-dead PI4KIIIβ-D656A or EGFP. Two days post-transfection, cells were infected with CVB3 or CVB3-Rluc in the presence of the indicated compounds. After lysing the cells at 8 h p.i., the total virus titer or the amount of luciferase activity was determined in the samples. (B, C) HeLa cells were co-transfected with plasmids encoding FAPP1-PH-GFP and either HA-tagged PI4KIIIβ-wt (B) or PI4KIIIβ-Y583M (C). The next day, cells were treated for 1 h with the indicated compounds. Subsequently, cells were fixed and the overexpressed PI4KIIIβ was stained using an antibody against HA. The percentage of Golgi-localized FAPP1-PH-GFP was determined by dividing the fluorescence intensity of the Golgi-area by that of the area of the whole cell. The resulting percentage was set to 100% in untreated cells. Bars represent the mean of 10 cells from three different experiments ± SD. Significant differences compared to untreated cells are indicated as follows: **P < 0.01, ***P < 0.001.