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. 2012 Sep 4;22(11):1576–1592. doi: 10.1038/cr.2012.129

Figure 7.

Figure 7

Replication of CVB3 3A mutants is largely independent of PI4KIIIβ. (A) HeLa cells were infected with wt CVB3 or mutant CVB3 3A-H57Y. At 5 h p.i., cells were fixed and stained with antibodies against PI4KIIIβ and dsRNA as a marker for the sites of RNA replication. (B, C) HeLa cells were transfected with siRNAs against PI4KIIIβ (B), or PI4KIIα, PI4KIIβ, and PI4KIIIα (C) and scrambled siRNA as a negative control. Two days later, siRNA-treated cells were infected with wt virus, 3A-V45A virus, or 3A-H57Y virus. Virus titers were determined at 8 h p.i. Bars represent mean of three samples ± SD, *P < 0.05. (D) In parallel, efficiency of the knock-down was determined by western blot analysis.