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CVB3 3A mutants do not require a high level of PI4P lipids. HeLa cells were infected with CVB3 wt or CVB3 3A-H57Y, after which the medium was replaced with (compound-containing) medium. At 5 h p.i., cells were fixed and stained with antibodies against 3A and PI4P. The PI4P to 3A fluorescence was quantified in 10 cells per condition and expressed as a ratio. Bars represent the mean ± SD. Significant differences compared to untreated cells are indicated as follows: ***P < 0.001.
