Figure 5.
A mechanism of spatiotemporal regulation of talin in mediating integrin activation. In unstimulated cells, talin is autoinhibited and hindered from accessing membrane via the charge-charge repulsion mechanism. Agonist stimulation promotes the co-localization of PIPKIγ with talin, thereby leading to the local enrichment of PIP2 for activating talin via the electrostatic pull-push mechanism. The electrostatic pull-push process is different from known steric occlusion by PIP2 that directly interferes with the autoinhibitory protein interfaces34,35.