Figure 3.

GC-MS analyses (in selected ion-monitoring mode) of products of carveol dehydrogenase assays using octakis-(6-O-methyl-2,3-di-O-pentyl)-γ-cyclodextrin as the chiral stationary phase. Ions monitored: for limonene, m/z 68, 93, and 136; for carvone, m/z 82, 108, and 150; for trans-carveol, m/z 84, 109, and 152; for cis-carveol, m/z 84, 109, and 134. A, Reference compounds. B through E, Products of carveol dehydrogenase activity with a (+)/(−)-trans-carveol mixture (B), a (+)/(−)-cis-carveol mixture (C), (−)-trans-carveol (D), and (−)-cis-carveol (E) as the substrates. For further details, see Methods.