Table II.
Dehydrogenase activity with various carveol isomers as substrates in 50 mm Gly buffer, pH 10.5, with 10% (v/v) glycerol, 2 mm DTT, and 1 mm each of NAD+ and NADP+
Substrate | Activity | Maximum |
---|---|---|
μm | nmol h−1 g−1 dry wt | % |
(+)/(−)-trans-Carveol (37/33) | 45.2 | 100 |
(+)/(−)-cis-Carveol (16/8) | 8.09 | 18 |
(−)-trans-Carveol (50) | 2.03 | 5 |
(−)-cis-Carveol (40) | 30.30 | 67 |