Figure 3.

Genetic modifications of the Notch pathway influenced N-cadherin and α9-integrin expression. Western blots showing the concomitant variations of Notch pathway activation (NICD and Hes1) and N-cadherin (N-Cad/FL and CTF2) and α9-integrin (ITGA9) expression in RMS cells transfected with Delta (A) or dnMAML1 (B). Below each western blot composition, a plot depicting densitometric analysis of each band of the western blot. The variations associated with genetic modification of the Notch pathway (Delta and MAML1 transfections) observed in protein are also seen in the mRNAs of N-cadherin, α9-integrin and Hes1 determined by quantitative PCR (C). All RNA measurements were taken in triplicate. Student’s t-test significance: *P<0.05, **P<0.005.