Figure 5.

Anti-N-cadherin- and anti-α9-integrin-blocking antibodies reduced invasive properties induced by Notch activation. Three RMS cell lines were transfected with the constructions indicated below the bottom of each bar (pCDNA, Delta) in the presence (+) or absence (−) of either N-cadherin-blocking antibody (N-Cad Ab) or α9-integrin-blocking antibody (α9-ITG Ab) as indicated in the figure. (A) Adhesion assay on fibronectin-coated plates. The percentage of cells attached was quantified per cell line and condition. (B) Wound-healing assay. All bars indicate the healing velocity of each cell line, transfection and treatment (C) Matrigel/Transwell assay. Invasiveness is represented as a percentage of cells in the lower compartment, compared with the control. The three assays were performed in three independent wells per cell line and condition. Student’s t-test significance: *P<0.05.