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Binding of NICD and Hes1 to N-cadherin and α9-integrin promoters. Lysates from RH30 cells were analysed by ChIP assay using specific antibodies against Hes1, NICD and rabbit IgG as a control. Immunoprecipitated chromatin was amplified by PCR using N-cadherin and α9-integrin promoter region-specific primers. IgG: negative control rabbit IgG; Input: 5% of the sonicated cell lysate used for IP; H2O: PCR negative control; M: 100 bp DNA ladder.
