Figure 3.

Expression pattern of the INCENP gene in primary NB and in in vitro NB models. (A) A shorter INCENP isoform comprising only exons 1–5 (NM_020238) was preferentially expressed in MYCN-amplified patients, whereas expression differences were less pronounced for the longer INCENP transcript NM_020238_2. Data are presented as log2 of mean expression in each group ±s.e.m. (B) Expression of INCENP exon 5 was significantly elevated in MYCN-amplified patients (MNA) when compared with patients with normal MYCN (MYCNsc), whereas expression of exon 15 was not (C). (D) Semiquantitative real-time PCR revealed that expression of INCENP exons 1–2 and 10–11 was upregulated in vitro in a MYCN-negative NB cell lines, SH-EP, upon MYCN overexpression (WAC2) or MYCN induction (SH-EP MYCN+).