Figure 4.

Differential transcript expression of the cell-cycle-associated genes, p15INK4B (CDKN2B) and cyclin B1 (CCNB1). (A) CDKN2B isoforms are differentially regulated between tumours with and without MYCN amplification, but not between tumours from patients with EFS and non-survivors (DoD). (B) A short isoform of CCNB1 (red line) is preferentially expressed in tumours from non-survivors (DoD). For both, A and B, data are presented as log2 of mean expression in each group ±s.e.m. (C) Higher expression of this shorter CCNB1 isoform was also detected in NB cell lines. (D) Inhibition of the CCNB1/Cdk1 interaction by a small molecule inhibitor induced apoptosis in these cell lines.