Skip to main content
. 2012 Sep 6;107(8):1423–1432. doi: 10.1038/bjc.2012.380

Figure 1.

Figure 1

Leprel4 and CRTAP are down-regulated by methylation in NHL. (A) RT–PCR and MSP analysis of expression and CpG island methylation of Leprel4 and CRTAP in NHL cell lines. Expression of Leprel4, CRTAP and GAPDH was determined as described in Materials and Methods. The MSP figure shows U and M specific reactions for the indicated NHL cell lines. Also shown is control methylated (CM) and control unmethylated (CU) genomic DNA modified in parallel with cell line DNA samples. (B) Pyrosequencing analysis of Leprel4 and CRTAP CpG islands in NHL cell lines. The % methylation at each analysed CpG dinucleotide is represented by the intensity of shading in the circles as shown in the figure, together with the mean % methylation for each cell line. (C) Demethylation reactivates expression of Leprel4 and CRTAP. MAK-I and AK31 cells (Leprel4 and CRTAP CpG islands methylated) were grown without AZA (−) or in the presence of AZA for 24 or 48 h as indicated and expression of Leprel4 and CRTAP determined by qPCR.