Figure 3.

Analysis of P4H genes in BL cell lines. (A) Pyrosequencing analysis of CpG island methylation in C-P4H genes in BL and LCL (B301, LCL-K, LCL3 and CR B95.8). Pyrosequencing was performed as described in Materials and Methods. The degree of methylation at each analysed CG dinucleotide is represented by the intensity of shading in the circles as shown in the figure. (B) RT–PCR analysis of P4H gene expression in BL cell lines. Expression of P4H genes and GAPDH was determined as described in Materials and Methods. (C) qPCR analysis of P4HA3 expression. Expression is shown relative to B301.