Skip to main content
. Author manuscript; available in PMC: 2013 Nov 7.
Published in final edited form as: J Am Chem Soc. 2012 Oct 26;134(44):18374–18379. doi: 10.1021/ja307315u

Table 1.

Rate constants for LThDP formation and decarboxylation on DXP synthase under pre-steady state conditionsa.

Reaction with DXP synthase k1 (s−1) k2 (s−1)
pyruvate, 6 °C 1.1 ± 0.03 n.d
pyruvate (single turnover, 6 °C) 1.4 ± 0.05 (7±0.01) × 10−2
pyruvate(single turnover, 37 °C) 2.8 ± 0.15 (4.9 ±0.01)×10−1
pyruvate+D-GAP,6°C (6.8±0.01)×10−1 (4.2 ± 1.0) × 101
a

See detailed conditions in Experimental Section; n.d. not determined; see Scheme 1 right for assignment of rate constants to individual steps.