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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2012 May 24;32(8):1884–1891. doi: 10.1161/ATVBAHA.112.250993

Figure 4. Histone neutralization is protective in ischemic stroke.

Figure 4

Wild-type mice treated with a histone-neutralizing antibody (BWA3) or isotype control antibody (IgG1) were subjected to 1 h of tMCAO and 23 h of reperfusion after which mice behavior was tested and brains analyzed. (A) Representative 2,3,5-triphenyl tetrazolium chloride stains of 3 consecutive coronal brain sections (left), on which ischemic infarctions appear white. Brain infarct volumes as measured by planimetry (right). Mice treated with BWA3 developed significantly smaller infarctions in comparison to mice treated with the isotype control (* p<0.05). (B) Functional Bederson and grip test scores. Neutralization of histones (BWA3) showed a tendency for a better neurologic outcome when compared to IgG1 control treatment. Treatment with vehicle gave the same results as treatment with IgG1 antibody (not shown, p=0.12).