Wild-type mice treated with histones or saline (vehicle) were subjected to 1 h of tMCAO and 23 h of reperfusion after which mice behavior was tested and brains analyzed. (A) Representative 2,3,5-triphenyl tetrazolium chloride stains of 3 consecutive coronal brain sections (left), on which ischemic infarctions appear white. Brain infarct volumes as measured by planimetry (right). Mice treated with histones developed significantly larger infarctions in comparison to mice treated with vehicle (* p<0.05). (B) Functional Bederson and grip test scores. Infusion of histones led to worsening of neurologic outcome, as seen by increased Bederson score, when compared to control treatment.