Table IV.

NaCl-induced GUS activity in R₁ leaves and roots of transgenic rice plants

NaCl Treatment	GUS Activity
	pJS105 Transgenic				pJS110 Transgenic
	L2		L5		L7		L11		NT
	Leaves	Roots	Leaves	Roots	Leaves	Roots	Leaves	Roots	Leaves	Roots
h	4-MU nmol h−1 mg−1 protein
0	0.9  ± 0.2	0.8  ± 0.2	6  ± 2	5  ± 1	13  ± 3	12  ± 2	10  ± 2	9  ± 2	0.02  ± 0.01	0.01  ± 0.01
48	0.9  ± 0.2	0.8  ± 0.2	10  ± 3	11  ± 3	20  ± 4	25  ± 4	16  ± 4	20  ± 4	0.02  ± 0.01	0.01  ± 0.01
72	0.9  ± 0.2	0.8  ± 0.2	14  ± 3	20  ± 5	28  ± 6	46  ± 6	21  ± 4	25  ± 5	0.02  ± 0.01	0.01  ± 0.01
96	0.9  ± 0.2	0.8  ± 0.2	17  ± 4	16  ± 3	59  ± 7	40  ± 4	28  ± 5	22  ± 3	0.02  ± 0.01	0.01  ± 0.01
96 h/0 h	1		3		4		3		1
72 h/0 h		1		4		4		3		1

Mean ± se values of NaCl-induced GUS activity were calculated from the results of three independent experiments and three plants were used for each experiment. Eight-week-old R₁ plants were grown in the greenhouse. After withholding water for 24 h, the third leaf or one-tenth the amount of roots was collected and used for a basal level test of GUS activity (0 h). Then, the plants were supplied with 150 mm NaCl solution. At 48, 72, and 96 h, the other three leaves or one-tenth the amount of roots were collected, respectively, and used for assaying NaCl-induced GUS activity. Maximum induction values are underlined. 96 h/0 h indicates the -fold induction of GUS activity in rice tissues after 96 h of treatment with 150 mm NaCl. 72 h/0 h indicates the induction. NT, Nontransgenic; 4-MU, 4-methylumbelliferone.