Table 1.
Specimens investigated with methods applied in addition to LM serial section examination
| Specimen number (spm) | Specimen length | Embedding medium | Section thickness | TEM | Number of LM sections used for subsequent TEM sectioning | Computerized 3D analysis (Amira software) |
|---|---|---|---|---|---|---|
| 3 |
415 μm |
Spurr’s lvr |
1.5 μm |
no |
- |
SR, total specimen |
| 3a |
504 μm |
Spurr’s lvr |
1.5 μm |
no |
- |
SR, metanephridial region |
| 4 |
513 μm |
Spurr’s lvr |
1.5 μm |
yes |
10 |
SR, metanephridial region |
| 4a |
522 μm |
Agar lvr |
1.5 μm |
yes |
2 |
aligned 3D stack |
| 4b |
558 μm |
Agar lvr |
1.5 μm |
|
- |
- |
| 5 |
579 μm |
Spurr’s lvr |
1.5 μm |
no |
- |
SR, total specimen |
| 6 |
580 μm |
Spurr’s lvr |
1.5 μm |
yes |
12 |
SR, metanephridial region |
| 7 |
604.5 μm |
Spurr’s lvr |
1.5 μm |
yes |
17 |
SR, total specimen |
| 7a |
594 μm |
Spurr’s lvr |
1.0 μm |
no |
- |
aligned 3D stack |
| 8 |
621 μm |
Spurr’s lvr |
1.5 μm |
yes |
14 |
SR, total specimen |
| 9 |
652.5 μm |
Spurr’s lvr |
1.5 μm |
yes |
7 |
aligned 3D stack |
| 10 |
753 μm |
Spurr’s lvr |
1.5 μm |
yes |
17 |
SR, total specimen |
| 11 |
~800 μm |
Spurr’s lvr |
1.5 μm / 1.0 μm |
yes |
- |
- |
| |
|
|
50–70 nm |
|
|
|
| 12 |
852 μm |
Spurr’s lvr |
1.5 μm |
no |
- |
SR, total specimen |
| 13 |
886.5 μm |
Spurr’s lvr |
1.5 μm |
yes |
12 |
SR, metanephridial region |
| 14 |
1159.5 μm |
Spurr’s lvr |
1.5 μm |
no |
- |
SR, total specimen |
| 15 |
1465.5 μm |
Agar lvr |
1.5 μm |
yes |
2 |
- |
| 16 | 1821 μm | Agar lvr | 1.5 μm | no | - | - |
Length specifications refer to embedded condition; lvr, low viscosity resin; SR, surface rendering.