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. 2012 Jun 13;9:110. doi: 10.1186/1743-422X-9-110

Table 2.

The cycle threshold and melting temperatures of GCV real-time PCR products

Sample Cycle treshold Melting temperature °C
Neg
 40.0
 -
Pos
 11.4
 80.2
1
 31.7
 80.0
2
 36.7
 79.9
3
 31.1
 80.0
4
 38.9
 80.1
5
 29.3
 79.9
6
 31.7
 80.0
7
 35.8
 80.2
8
 28.6
 79.9
9
 37.9
 80.2
10
 36.8
 80.0
11
 35.9
 80.0
12
 40.0
 -
13
 32.0
 80.0
14
 36.1
 80.0
15
 34.6
 80.2
16
 30.9
 80.2
17
 34.6
 80.4
18
 32.0
 80.6
19
 21.6
 80.2
20
 38.1
 80.3
21
 30.7
 80.4
22
 36.4
 80.2
23
 19.3
 80.4
24
 34.2
 80.4
25
 35.9
 80.4
26
 35.6
 80.4
27
 34.2
 80.2
28
 31.2
 80.2
29
 20.4
 80.2
30
 27.4
 79.9
31
 26.7
 80.4
32
 27.1
 80.4
33
 29.2
 80.6
34
 37.0
 80.6
35
 26.1
 80.6
36
 33.7
 80.8
37
 36.3
 80.4
38 30.6 80.8

The values of cycle threshold (CT) and melting temperature in °C were given in the table.