Figure 1. Actin filament disruption prevented the autophagic response upon starvation or rapamycin treatment. (A) CHO cells stable expressing EGFP-MAP1LC3 were incubated for 2 h at 37°C in full nutrient medium (Ctrl) (a, d, c and f) or starvation medium (Stv) (b and e); in the presence (d–f) or absence (a–c) of the actin depolymerizing agent Latrunculin B (LatB) (10 μM), and/or 100 μM of Rapamycin (Rap) (b and e). Subsequently, cells were fixed in 3% paraformaldehyde and processed for immunofluorescence. (B) The EGFP-MAP1LC3 dots were quantified from max intensity projection of a confocal z-stack and the mean+SEM of the number of dots per cell is shown. Data evaluated correspond to three independent experiments. Scale bars: 10 μm.