Figure 2. Upregulation of IRGM1 in neuron after pMCAO. RNA and protein sample were isolated either from contra-lateral or lpsi-lateral of the cortex at different time point after pMCAO. (A) Changes of Irgm1 at mRNA level was detected by real-time PCR, (p < 0.05 compare to sham, n = 5/group). (B) Changes of IRGM1 at protein level were measured by western blot. ACTB was used as the loading control, (n = 3/group). “+” means ischemic side; “-” means nonischemic side. (C) The immunofluorescence staining was used to locate IRGM1 (red) expression 24 h after pMCAO. RBFOX3/NeuN (green) was used as the marker for neurons. Data represent 3 independent experiments. (D) mRNA level of Ifng was detected by real-time PCR (p < 0.05, n = 4/group).