Figure 3. Deficiency of autophagy in Irgm1 knockout mice. (A) The basal level of LC3-I, LC3-II and SQSTM1/p62 was measured by western blot. (B) TEM showed lower autophagy vacuoles were found in Irgm1^–/– mice after pMCAO (upper panel). Neuron autophagy observed by TEM was quantified by either percentage of AV positive neurons (left) or the total number of AV (right). Data represent 4 mice per group, 3 section per mice; 20 fields/section. p < 0.05. (C) LC3-I and LC3-II expression was detected by western blot (upper panel). Comparison was made between Irgm1^+/+ and Irgm1^–/– mice in terms of their LC3-II/LC3-I ratio (low panel, p < 0.05, n = 4/group). (D) Expression of SQSTM1/p62 in Irgm1^+/+ and Irgm1^–/– mice 24 h after pMCAO. ACTB was used as the loading control. Bar represents mean ± SEM from 4 mice in each group.*p < 0.05 compared with Irgm1^+/+ group. ‘+’: ischemic side; ‘-’: nonischemic side.