Figure 4. Increase of neuron necrosis in Irgm1 KO mice after pMCAO. (A) TTC staining was used to measure the volume of the infarct area (5–6 mice/group. p < 0.05). (B) Semi-thin sections were stained with basic fuchsin to show cell structure after pMCAO in both wild-type and Irgm1 KO mice (scale bar: 50 µm). (C) Example data of TEM shows neuron apoptosis and necrosis after pMCAO in wild-type and Irgm1 KO mice. (D) Cleaved CASP3 expression at 24 h after pMCAO in either Irgm1^+/+ or Irgm1^–/– mice was determined by western blot (p < 0.05, n = 4). (E) Immuno-fluorescence staining to quantify and compare CASP3-positive neurons between Irgm1^+/+ and Irgm1^–/– mice (p < 0.05, n = 4).