Figure 3. Deficiency of CD47 increases the autophagic flux. WT and CD47-deficient Jurkat T cells were cultured in complete medium and exposed to a 10 Gy dose of ionizing radiation. Cells were harvested at 0, 2, 6 and 24 h after IR. Protein expression was assessed by western blot hybridization using specific antibodies to LC3 (A) and SQSTM1 (B). Data were quantified by densitometry and normalizing the signal to that of ACTB/β-actin in the respective cells (n = 3; one-way ANOVA-Bonferroni post-test, *p < 0.05).