Figure 5. Pharmacological inhibition of autophagy sensitizes CD47-deficient Jurkat T cells to IR. WT and CD47-deficient Jurkat T cells were plated in complete medium in 96-well plates. One hour prior to IR, cells were treated with 25 µM hydroxychloroquine (A) or 5 mM 3-MA (B). Cell viability was measured using MTS reagent after 72 h (n = 3 in triplicate, one-way ANOVA-Bonferroni post-test *p < 0.0001)