Figure 8. Re-expression of CD47 in CD47⁻ Jurkat cells reverses radioprotection and reduces the autophagic flux. (A) Flow cytometry analysis using FITC-conjugated CD47 antibody B6H12 demonstrates re-expression of CD47 in CD47⁻ Jurkat cells. (B) Western blot hybridization analysis of CD47 expression in Jurkat, CD47⁻, and CD47⁻ transfected with a plasmid expressing CD47. (C) Re-expression of CD47 in CD47⁻ cells sensitizes this cell line to IR as measured by MTS assay (n = 3 one way ANOVA, *p < 0.04). (D) WT, CD47⁻ and CD47⁻ + CD47 and were cultured in complete medium and harvested before and after exposure to a 10 Gy dose of IR. Protein expression was assessed by western blot hybridization using LC3 and SQSTM1 antibodies. (E) Representative confocal images and quantitative analysis show LC3 puncta formation of cells transfected with GFP-LC3.