Figure 3. HA-specific CD8⁺ and CD4⁺ T-cell proliferation in the draining nodes pre- and post-complete AB1HA tumor resection. (A) CL4 CD8⁺ T cells were transferred at day 16 of tumor growth (day -1), at the time of surgery (day 0), or on post-operative days: 4,7,14, and 28. Each data points depicts proportion of proliferating CFSE⁺ CL4 T cells detected in pooled inguinal and axillary DLN from an individual mouse. Mean proliferation of group indicated by bars. (B) HNT proliferation was assayed in BALB/c with established (day 16) AB1HA tumors, and two weeks after surgery. Experiment was performed once, with five animals in the pre-operative and 2 week post-operative groups (n = 10 for naïve group). Each data points depicts proportion of proliferating CFSE⁺ HNT T cells detected in pooled inguinal and axillary DLN from an individual mouse.