Figure 3. Discrimination of the BDD OfVIII Heavy and Light Chains.

Immunoprecipitation using domain specific MAbs was performed by incubation with ofVIII in the presence and absence of thrombin. Heavy and light chains were dissociated using M-PER lysis buffer supplemented with 150 mM NaCl prior to MAb addition. FVIII heavy chain was precipitated with 4F4 1B, an A2 domain-specific mAb, and light chain was precipitated with I14 1B, a C2 domain-specific mAb. MAbs incubated with vehicle served as negative controls.