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. 2012 Nov 9;7(11):e49481. doi: 10.1371/journal.pone.0049481

Figure 4. Thrombin-Activated Decay Rate of OfVIIIa.

Figure 4

Human (closed circle) and ovine (open circle) fVIIIa decay was measured by chromogenic Xase assay in which 20 nM fVIII was activated with thrombin and then stopped with desulfatohirudin. Activated fVIIIa in complex with phospholipid vesicles, activated factor IXa, and factor X was measured at 0.5, 3, 5, 8, 15, and 30 minutes to determine residual fVIIIa activity. Half-lives of 1.8±0.09 and 3.5±0.37 minutes were calculated for human and ovine fVIIIa, respectively. Data shown represents the percent of initial activity by semi-log extrapolation to time  = 0. Regression analysis revealed Pearson correlation coefficients of 0.999 for both treatments.