Figure 5. BDD OfVIII Binding to VWF.

Kinetic ELISA was conducted using human VWF to capture human (closed circles) or ovine (open circles) fVIII. Plates were coated with 50 µl of 6 mg/ml human VWF and blocked with 2% BSA. Monoclonal A2 domain fVIII antibody 4F4 1B was added to each well and colorimetric transmission was activated with para-nitrophenylphosphate substrate following streptavidin alkaline phosphatase. Data shown are the mean of three independent experiments ± sample standard deviation.