Table 1. Purification of BDD OfVIII.
Sample | Vol. (ml) | A280 | Total A280 | Activity (U/ml) | Units | Units/A280 | AQ | % Yield | Fold Pur. |
Media | 5,350 | 1.53 | 8,186 | 3.78 | 20,223 | 2.47 | 28 | 100 | 1 |
SP-Sepharose pool | 35 | 0.415 | 14.5 | 353.4 | 12,370 | 853 | 49 | 61 | 345 |
Source Q pool | 2.4 | 0.211 | 0.506 | 2,149 | 5,158 | 10,193 | 55 | 25.5 | 4,126 |