Table 4. Results of replicability test of AllGlo quadruplex qPCR.
Concentration* | HPV6 | HPV11 | HPV16 | HPV18 | ||||
Intra# | Inter | Intra | Inter | Intra | Inter | Intra | Inter | |
102 | <1.1 | 1.43 | <1.16 | 0.90 | <1.31 | 1.00 | <1.39 | 1.02 |
104 | <0.86 | 0.78 | <1.25 | 0.95 | <1.27 | 0.96 | <1.46 | 1.23 |
106 | <1.21 | 0.80 | <1.07 | 0.79 | <1.21 | 0.99 | <1.26 | 0.86 |
108 | <1.17 | 0.97 | <0.92 | 0.72 | <1.96 | 1.12 | <1.16 | 0.95 |
Five side-by-side AllGlo quadruplex quantitative PCR assay were carried out using the DNA aliquots in triplicate to determine the mean CT value, and the mean CT value was used for statistics of coefficient of variation (%) for four HPV types.
copies/test.
Intra-assay coefficient variation.
Inter-assay coefficient variation.