Figure 2. Replacement of AA residues with functionally equivalent residues restores the properties of NFL-TBS.40-63.

Using a similar experimental approach to that described in Figure 1, the amino-acids at position 1, 12, 14 and 16 were replaced by amino-acids with similar properties. While substitution of these AAs by alanine abolished their properties, replacement of these AAs by functionally equivalent residues restore the capacity to penetrate in cells and to alter the MT cytoskeleton. As in Figure 1A, experiments were triplicated and a minimum of 200 cells was analyzed in each experiment.