| NFL-TBS.40-63 |
Biotin-YSSYSAPVSSSLSVRRSYSSSSGS-CONH2
|
2712.4 |
10.3 |
−0.1 |
51.2±4.2 |
58.4±2.3 |
|
Alanine scan
|
| Tyr1Ala |
Biotin-
ASSYSAPVSSSLSVRRSYSSSSGS-CONH2
|
2620.1 |
11.2 |
0 |
0 |
24.1±4.6 |
| Ser2Ala |
Biotin-YASYSAPVSSSLSVRRSYSSSSGS-CONH2
|
2696.4 |
10.3 |
−0.1 |
5.9±6.4 |
23.5±1.1 |
| Ser3Ala |
Biotin-YSAYSAPVSSSLSVRRSYSSSSGS-CONH2
|
2696.4 |
10.3 |
−0.1 |
0.8±1.2 |
19.1±0.9 |
| Tyr4Ala |
Biotin-YSSASAPVSSSLSVRRSYSSSSGS-CONH2
|
2620.4 |
11.2 |
0 |
5.3±2.4 |
24.4±4.6 |
| Ser5Ala |
Biotin-YSSYAAPVSSSLSVRRSYSSSSGS-CONH2
|
2696.4 |
10.3 |
−0.1 |
16.0±1.4 |
23.6±1.6 |
| Ala6Ala |
Biotin-YSSYSAPVSSSLSVRRSYSSSSGS-CONH2
|
2712.4 |
10.3 |
−0.1 |
50.3±4.6 |
48.1±6.6 |
| Pro7Ala |
Biotin-YSSYSAAVSSSLSVRRSYSSSSGS-CONH2
|
2686.4 |
10.3 |
−0.1 |
16.7±4.6 |
26.6±5.9 |
| Val8Ala |
Biotin-YSSYSAPASSSLSVRRSYSSSSGS-CONH2
|
2684.4 |
10.3 |
−0.1 |
9.9±3.4 |
23.6±2.1 |
| Ser9Ala |
Biotin-YSSYSAPVASSLSVRRSYSSSSGS-CONH2
|
2696.4 |
10.3 |
−0.1 |
23.3±8.4 |
27.1±4.8 |
| Ser10Ala |
Biotin-YSSYSAPVSASLSVRRSYSSSSGS-CONH2
|
2696.4 |
10.3 |
−0.1 |
34.5±0 |
32.9±1.9 |
| Ser11Ala |
Biotin-YSSYSAPVSSALSVRRSYSSSSGS-CONH2
|
2696.4 |
10.3 |
−0.1 |
2.5±2.5 |
15.9±6.5 |
| Leu12Ala |
Biotin-YSSYSAPVSSSASVRRSYSSSSGS-CONH2
|
2670.4 |
10.3 |
0 |
0 |
15.5±2.8 |
| Ser13Ala |
Biotin-YSSYSAPVSSSLAVRRSYSSSSGS-CONH2
|
2696.4 |
10.3 |
−0.1 |
12.2±0.8 |
16.6±5.2 |
| Val14Ala |
Biotin-YSSYSAPVSSSLSARRSYSSSSGS-CONH2
|
2684.4 |
10.3 |
−0.1 |
0 |
13.8±3.9 |
| Arg15Ala |
Biotin-YSSYSAPVSSSLSVARSYSSSSGS-CONH2
|
2627.3 |
9.7 |
−0.2 |
18.1±1.3 |
25.5±6.3 |
| Arg16Ala |
Biotin-YSSYSAPVSSSLSVRASYSSSSGS-CONH2
|
2627.3 |
9.7 |
−0.2 |
0 |
15.6±3.0 |
| Ser17Ala |
Biotin-YSSYSAPVSSSLSVRRAYSSSSGS-CONH2
|
2696.4 |
10.3 |
−0.1 |
4.6±1.8 |
18.1±4.4 |
| Tyr18Ala |
Biotin-YSSYSAPVSSSLSVRRSASSSSGS-CONH2
|
2620.4 |
11.2 |
0 |
7.9±0.9 |
16.0±2.8 |
| Ser19Ala |
Biotin-YSSYSAPVSSSLSVRRSYASSSGS-CONH2
|
2696.4 |
10.3 |
−0.1 |
5.2±0.7 |
14.5±2.7 |
| Ser20Ala |
Biotin-YSSYSAPVSSSLSVRRSYSASSGS-CONH2
|
2696.4 |
10.3 |
−0.1 |
6.4±1.2 |
16.4±6.4 |
| Ser21Ala |
Biotin-YSSYSAPVSSSLSVRRSYSSASGS-CONH2
|
2696.4 |
10.3 |
−0.1 |
55.4±5.9 |
31.6±5.1 |
| Ser22Ala |
Biotin-YSSYSAPVSSSLSVRRSYSSSAGS-CONH2
|
2796.4 |
10.3 |
−0.1 |
19.7±5.0 |
24.1±1.0 |
| Gly23Ala |
Biotin-YSSYSAPVSSSLSVRRSYSSSSAS-CONH2
|
2726.4 |
10.3 |
−0.1 |
6.2±6.2 |
16.9±4.5 |
| Ser24Ala |
Biotin-YSSYSAPVSSSLSVRRSYSSSSGA
-CONH2
|
2696.4 |
10.3 |
−0.1 |
44.3±6.7 |
29.4±0.6 |
|
Substitutions with functionally equivalent amino acids
|
| Tyr1Phe |
Biotin-
FSSYSAPVSSSLSVRRSYSSSSGS-CONH2
|
2696.4 |
11.2 |
−0.1 |
56.3±2.8 |
41.2±4.0 |
| Leu12Val |
Biotin-YSSYSAPVSSSVSVRRSYSSSSGS-CONH2
|
2698.4 |
10.3 |
−0.1 |
58.6±5.0 |
43.8±1.7 |
| Val14Leu |
Biotin-YSSYSAPVSSSLSLRRSYSSSSGS-CONH2
|
2726.4 |
10.3 |
−0.1 |
44.2±2.2 |
39.2±2.4 |
| Arg16Lys |
Biotin-YSSYSAPVSSSLSVRKSYSSSSGS-CONH2
|
2684.4 |
10.1 |
−0.1 |
32.9±1.3 |
36.9±0.9 |
