Table 2. Sequences of reverse (NFL-SBT) and scrambled (NFL-SCR) peptides, as well as the phosphorylated peptides.
| Peptide | Sequence | Molecular weight (g/mol) | PI | Average hydrophilicity | Peptide incorporation (% of cells) | Disintegration of MT (% of cells) |
| NFL-TBS.40-63 | Biotin-YSSYSAPVSSSLSVRRSYSSSSGS-CONH2 | 2712.4 | 10.3 | −0.1 | 51.2±4.2 | 58.4±2.3 |
| Scrambled peptides | ||||||
| NFL-SBT | Biotin-SGSSSSYSRRVSLSSSVPASYSSY-CONH2 | 2712.4 | 10.3 | −0.1 | 0 | 32.41±0.87 |
| NFL-SCR | Biotin-SLGSPSSSVRASYSSSRSYVYSSS-CONH2 | 2712.4 | 10.3 | −0.1 | 64.2±4.2 | 18.18±3.2 |
| Phosphorylated and acid aspartic peptides | ||||||
| Ser17SerP | Biotin-YSSYSAPVSSSLSVRRSpYSSSSGS-CONH2 | 2792.4 | 10.3 | −0.1 | 32.8±8.7 | 22.9±1.3 |
| Ser19SerP | Biotin-YSSYSAPVSSSLSVRRSYSpSSSGS-CONH2 | 2792.4 | 10.3 | −0.1 | 27.3±12.1 | 19.4±2.1 |
| Ser17,19SerP | Biotin-YSSYSAPVSSSLSVRRSpYSpSSSGS-CONH2 | 2872.4 | 10.3 | −0.1 | 32±2.1 | 11.0±3.0 |
| Ser17Asp | Biotin-YSSYSAPVSSSLSVRRDYSSSSGS-CONH2 | 2740.4 | 9.7 | 0 | 31.3±11.2 | 22.2±3.4 |
| Ser19Asp | Biotin-YSSYSAPVSSSLSVRRSYDSSSGS-CONH2 | 2740.4 | 9.7 | 0 | 25.2±4.8 | 19.2±1.5 |
| Ser17,19Asp | Biotin-YSSYSAPVSSSLSVRRDYDSSSGS-CONH2 | 2768.4 | 6.8 | 0.1 | 29.0±12.7 | 18.6±6.8 |