Figure 2. Western blot analysis of the HIS-tagged human recombinant αTFP and MLCL AT activity of the HIS-tagged human recombinant αTFP in presence of palmitoyl-Coenzyme A or oleoyl-Coenzyme A or linoleoyl-Coenzme A.

A. HIS-tagged human recombinant αTFP was generated, purified and 2 µg protein subjected to SDS-PAGE and then western blot analysis performed using the polyclonal antibody to MLCL AT-1 as described in Materials and Methods. A representative blot is shown. Molecular mass markers are indicated on the left. Arrow indicates the HIS-tagged recombinant αTFP. B. MLCL AT in vitro activity of purified recombinant αTFP was determined in presence of [1-¹⁴C]palmitoyl-Coenzyme A (C16∶0) or [1-¹⁴C]oleoyl-Coenzyme A (C18∶1) or [1-¹⁴C]linoleoyl-Coenzme A (C18∶2) as described in Materials and Methods. Data represent the mean ± standard deviation of three experiments. HIS-tagged human recombinant αTFP was generated, purified and MLCL AT in vitro activity was determined in the presence of MLCL and various concentrations of linoleoyl-Coezyme A (C) or oleoyl-Coenzyme A (D) or palmitoyl-Coenzyme A (E) as described in Materials and Methods. Data represent the mean of two experiments assayed in duplicate.