Figure 3. Western blot analysis of αTFP and incorporation of fatty acids into CL in HeLa cells transfected with human recombinant αTFP.

HeLa cells (A) were mock transfected (Mock) or transfected with human recombinant αTFP plasmid (Plasmid) and mitochondrial fractions prepared and 25 µg protein subjected to SDS-PAGE followed by western blot analysis using anti-αTFP antibody or anti-β-actin antibody as described in Materials and Methods. A representative blot is depicted. Hela cells (B) were mock transfected (mock) or transfected with human recombinant αTFP (alpha) and incubated with for 4 h with [1-¹⁴C]linoleate (linoleate) or [1-¹⁴C]oleate (oleate) or [³H]palmitate (palmitate) and radioactivity incorporated into CL determined. Data represent the mean ± standard deviation of three experiments. *p<0.05.