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Sema3A is also known to suppress VEGF-induced neovascularization. In order to examine possible angiogenesis by inhibiting Sema3A, neovascularization in the transplanted grafts were quantified by anti-CD31 immunostaining (A, B) and image tracing (C, D). There was no significant difference in vascular area between the two groups (E). Scale bar = 500 µm in A, B.
