Figure 5. SM-345431 inhibits epithelial cell proliferation but does not affect cell viability or Sema3A production.

Since the corneal epithelium was the predominant source of Sema3A in the cornea, the effect of SM-345431 on cultured murine corneal epithelial cells were observed. There was a slight dose-dependent inhibition on cell proliferation (A) (n = 5, *, **P<0.05, One way ANOVA followed with Scheffe's F test). However, there was no effect on Sema3A production observed by western blot (B, C) or on cell viability under the dose used in the in vivo experiments.