. Author manuscript; available in PMC: 2014 Feb 1.

Published in final edited form as: J Biomed Mater Res A. 2012 Aug 3;101(2):456–464. doi: 10.1002/jbm.a.34353

Table 1.

Comparison of protein purification methods: yield and endotoxin level. Protein yield was calculated spectrophotometrically at A₂₈₀ and indicated as the purified protein amount per liter of culture medium. Endotoxin level was measured using LAL Kinetic-QCL assay. 1 EU=Endotoxin Unit corresponds 100 pg of endotoxin/lipopolysaccharide (LPS). ND- not determined.

	15X80/20	15XPA	6XPA
Protein yield (mg/L)	45.8	200	96.8
Endotoxin after dialysis	<1 EU/ml	3.48 EU/ml	2.44 EU/ml
Endotoxin after IEX	ND	<1 EU/ml	1.30 EU/ml 4 EU/mg
Endotoxin after polishing	<1 EU/ml	<1 EU/ml	1.35 EU/ml 2.5 EU/mg