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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Mol Cell Neurosci. 2012 Aug 27;51(3-4):89–100. doi: 10.1016/j.mcn.2012.08.010

Figure 5.

Figure 5

Presynaptic signaling acts non-cell autonomously to down-regulate DNlg1 levels during overgrowth. A–E) Confocal projections of NMJs from synapse overgrowth genotypes and controls labeled with anti-DNlg1 (green) and anti-HRP (red). DNlg1 staining (single channel) is shown in panels F – J. Activity-dependent signaling down regulates dnlg1 expression. Synaptic DNlg1 levels are reduced in eag1Sh14 (G) compared to wild-type controls (F), and following neural overexpression of AP-1 in elav-Gal4; UAS-AP1 NMJs (J) compared to elav-Gal4 controls (I). hiw signaling is required for normal levels of dnlg1 expression. DNlg1 levels are reduced at hiwΔN mutant synapses (H) compared to controls (F). K) Western blot of larval body wall extracts from wildtype, dnlg1− and two overgrowth mutants probed with anti-DNlg1 antisera. A polypeptide of molecular weight 150kD (arrow) is detected in wildtype that is absent in dnlg1Δ46/ Df and reduced in eag1 Sh14 and hiwΔN mutants. L) dnlg1 RNA levels measured by Q-PCR are reduced in body walls from eag1 Sh14 as compared to wild-type controls.