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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Cancer Lett. 2012 Sep 23;328(1):55–64. doi: 10.1016/j.canlet.2012.09.011

Fig. 6.

Fig. 6

FN1 is involved in the TIP-1-regulated angiogenesis in human glioblastomas. (A) D54 cells with or without TIP-1 knockdown were mixed with 25 μg/ml of recombinant FN1 protein or PBS control for the matrigel-plug assays. Tumor blood vessels within the matrigel-plugs were detected and quantified at day 8 after plug implantation as described in Materials and Methods. p<0.05, n=5. The Student’s t-test. (B) FN1 expression within D54 cells was down-regulated with two validated siRNAs against human FN1 transcripts. The efficiency of siRNA was detected with western blot analyses with specific antibodies (lane 1, control siRNA; lane 2, FN1 siRNA #1; and lane 3, FN1 siRNA #2) as shown in the insert. The transfected cells were used for the matrigel-plug assays 72 hours post-transfection. Shown are representative images and quantitative measurements of fluorescence intensity (ImageJ). * p<0.05 compared to controls by the Student’s t-test.