Figure 2.

RC-101 inhibits G. vaginalis de novo biofilm formation but does not break down established biofilms or inhibit planktonic growth. (a) Bacteria were grown for 24 h in the presence of vehicle control or RC-101 at the indicated concentrations and biofilm formation determined by safranin staining, solubilization and measurement of OD₄₅₀. (b) G. vaginalis biofilms were grown for 24 h without treatment and then exposed to vehicle control or RC-101 at the indicated concentrations for an additional 24 h prior to staining. (c) G. vaginalis was exposed to RC-101 or vehicle control for 1 or 3 h and bacterial concentration determined by quantitative culture. (d) G. vaginalis growth was measured by monitoring OD₅₉₀ in the presence of RC-101 (final concentration 12.5 mg/L) or vehicle control. *P < 0.05; ***P < 0.001.