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Inhibition of relaxation activity of EcTOPI by DMA. Lane 1, negatively supercoiled pBAD/Thio plasmid DNA; lane 2, relaxed plasmid DNA by treatment with EcTOPI; lanes 3–10, plasmid DNA + EcTOPI in the presence of 1, 5, 7.5, 10, 12.5, 15, 20 and 30 μM DMA, respectively. FR, fully relaxed; PR, partially relaxed; OC, open circle; SC, supercoiled. The percentage inhibition values were averages obtained from three independent experiments. Error bars denote standard deviations.
