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. 2012 Sep 3;67(12):2882–2891. doi: 10.1093/jac/dks322

Figure 6.

Figure 6.

Relaxation of pBAD/Thio by human topoisomerase II α in the presence of DMA. (a) Lane 1, negatively supercoiled pBAD/Thio plasmid DNA; lane 2, relaxation of pBAD/Thio by human topoisomerase II α; lanes 3–10; pBAD/Thio + human topoisomerase II α in the presence of 1, 5, 7.5, 10, 12.5, 15, 20 and 30 μM DMA, respectively. HuTOPIIα, human topoisomerase II α; FR, fully relaxed; PR, partially relaxed; OC, open circle; SC, supercoiled. The percentage inhibition values were averages obtained from three independent experiments. Error bars denote standard deviations. (b) Decatenation of kDNA with human topoisomerase II α in the presence of DMA. Lane 1, kDNA as control; lane 2; decatenation of kDNA by human topoisomerase II α, lane 3; kDNA with 100 μM DMA, lanes 4–10; kDNA + human topoisomerase II α in the presence of 1, 5, 10, 25, 50, 75 and 100 μM DMA, respectively. HuTOPIIα, human topoisomerase II α; N, nicked monomer; C, closed monomer.