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. 2012 Nov 12;199(4):613–622. doi: 10.1083/jcb.201207079

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© 2012 Bui et al.

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Figure 2. — Dnm1 InsB conserved residues are important for Dnm1 function in mitochondrial fission. (A) Quantification of mitochondrial morphology in dnm1Δ cells expressing indicated Dnm1 variants. (B) Lysates from cells expressing the indicated C-terminal HA- or Myc-tagged Dnm1 proteins were used for immunoprecipitation with anti-Myc agarose beads. Immunoprecipitated fractions (top) and lysates (bottom) were analyzed by SDS-PAGE and Western blotting with anti-Myc and anti-HA antibodies. (C) Quantification of mitochondrial morphology in WT cells expressing indicated Dnm1 variants. (A and C) Black columns and error bars represent the mean and standard deviation of at least three independent experiments (n = 100). (D) pGBD and pGAD plasmids expressing the indicated fusion proteins were cotransformed into the Y187 yeast two-hybrid reporter strain and grown on S-Dextrose minus adenine plates at 30°C for 3 d.