Table 1.
Mutations | WT mitochondrial morphologya | GFP mitochondrial punctab | Interaction with Dnm1c | |||
DNM1 mdv1Δ | dnm1F610A mdv1Δ | Dnm1 | Dnm1F610A | Dnm1 | Dnm1F610A | |
% | % | |||||
Mdv1 | 67 ± 7 | 0 ± 0 | + | + | + | − |
Y418C | 6 ± 3 | 41 ± 16 | + | + | ++ | + |
K430R | 9 ± 8 | 28 ± 4 | + | + | + | − |
E504G | 19 ± 8 | 32 ± 16 | + | + | ++ | − |
D539G | 9 ± 2 | 68 ± 11 | + | + | ++ | + |
D539Y | 11 ± 1 | 61 ± 14 | + | + | ++ | + |
L540P | 12 ± 5 | 58 ± 14 | + | + | ++ | + |
S541G | 8 ± 4 | 38 ± 12 | + | + | ++ | + |
S557C | 9 ± 8 | 45 ± 8 | + | + | ++ | + |
T558I | 10 ± 8 | 34 ± 6 | + | + | ++ | + |
D576G | 68 ± 17 | 53 ± 9 | + | + | − | − |
D585G | 45 ± 3 | 30 ± 8 | + | + | − | − |
D607G | 15 ± 6 | 31 ± 12 | + | + | ++ | − |
Numbers are the mean and standard deviation of at least three independent experiments, n = 300.
+, GFP puncta localized to mitochondrial tubules.
Yeast two-hybrid assays. −, No growth on His or Ade minus medium; +, growth on His but not Ade minus medium; ++, growth on both His and Ade minus media. Growth on Ade minus medium is the more stringent indicator of protein–protein interaction.