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. 2012 Nov 12;199(4):589–598. doi: 10.1083/jcb.201203150

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© 2012 Li et al.

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Figure 1. — UBC-9 interacts with and SUMOylates ARL-13. (A, top) GST pull-down assay with GST-tagged ARL-13 full-length or PRD and His-tagged UBC-9 protein. (A, bottom) Ponceau S staining. (B) ARL-13 and UBC-9 label the middle segments but not the distal segments of cilia. Arrows with solid lines, ciliary base; arrows with broken lines, middle-distal junction; arrowheads, ciliary tip. Bar, 3 µm. (C) Predicted ARL-13 SUMOylation sites (above) and in vitro SUMOylation assay results (below). (top) Western blot with anti-SUMO antibody. (bottom) Ponceau S staining.